منابع مشابه
RNA-primed PCR.
We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of th...
متن کاملFibroblast phenotype in male carriers of FMR1 premutation alleles.
Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult-onset neurodegenerative disorder among carriers of premutation expansions (55-200 CGG repeats) of the fragile X mental retardation 1 (FMR1) gene. The clinical features of FXTAS, as well as various forms of clinical involvement in carriers without FXTAS, are thought to arise through a direct toxic gain of function of high levels of ...
متن کاملScreen for excess FMR1 premutation alleles among males with parkinsonism.
BACKGROUND Individuals with fragile X-associated tremor/ataxia syndrome frequently have associated features of parkinsonism, often leading to an initial diagnosis of Parkinson disease or other parkinsonism spectrum disorders. Parkinson disease populations may thus include individuals who harbor premutation expansions (55-200 CGG repeats) of the fragile X mental retardation 1 (FMR1) gene. OBJE...
متن کاملWhole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR).
INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. Unlike other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable to DNA ex...
متن کاملGene walking by unpredictably primed PCR.
Several methods have been described for gene walking by PCR on uncloned DNA (2 — 13). In general, they suffer from limitations: they either avoid high-complexity whole genomes, require delicate manipulation or give contaminated products. This report describes a reliable and simple PCR (1) adaptation for gene walks from any specific genomic locus: unpredictably primed PCR (UP-PCR). Besides its s...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2014
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2014.06.006